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Fernandez, Timea Gerczei. Biochemistry Laboratory Manual For Undergraduates.: De Gruyter, 2015. — 1 online resource — <URL:http://elib.fa.ru/ebsco/1805212.pdf>.Record create date: 3/11/2015 Subject: Biochemistry — Laboratory manuals.; Molecular biology — Laboratory manuals.; Drug resistance in microorganisms — Laboratory manuals. Collections: EBSCO Allowed Actions: –
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Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
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Table of Contents
- Preface
- Contents
- Introducing the Bacterial Antibiotic Sensor Mini Project
- Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are I
- Designing Primers for Site-Directed Mutagenesis
- Performing Site-Directed Mutagenesis
- Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel
- Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
- Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
- Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV s
- Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluo
- Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
- Understanding the Importance of Buffers in Biological Systems
- Molecular Visualization of an Enzyme, Acetylcholinesterase
- Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experimen
- Separation of the Phosphatidylcholines Using Reverse Phase HPLC
- List of Figures
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