"This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology"--Provided by publisher.

• Preface
• Foreword
• Contents
• List of contributing authors
• Part I: Basics
• 1 Brief history of fluorescence lifetime imaging
• 2 The long journey to the laser and its use for nonlinear optics
• 3 Advanced TCSPC-FLIM techniques
• 4 Ultrafast lasers in biophotonics
• Part II: Modern nonlinear microscopy of live cells
• 5 STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities
• 6 Principles and applications of temporal-focusing wide-field two-photon microscopy
• 7 FLIM-FRET microscopy
• 8 TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing
• 9 Laser tweezers are sources of two-photon effects
• 10 Metabolic shifts in cell proliferation and differentiation
• 11 Femtosecond laser nanoprocessing
• 12 Cryomultiphoton imaging
• Part III: Nonlinear tissue imaging
• 13 Multiphoton Tomography (MPT)
• 14 Clinical multimodal CARS imaging
• 15 In vivo multiphoton microscopy of human skin
• 16 Two-photon microscopy and fluorescence lifetime imaging of the cornea
• 17 Multiscale correlative imaging of the brain
• 18 Revealing interaction of dyes and nanomaterials by multiphoton imaging
• 19 Multiphoton FLIM in cosmetic clinical research
• 20 Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery
• 21 Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models
• 22 Bedside assessment of multiphoton tomography
• Index