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Multiphoton microscopy and fluorescence lifetime imaging: applications in biology and medicine / edited by Karsten König. — 1 online resource (480 pages). — EbpS Open Access. — English. — <URL:http://elib.fa.ru/ebsco/2944942.pdf>.Record create date: 1/27/2018 Subject: Fluorescence microscopy.; Microscopy, Fluorescence, Multiphoton.; Optical Imaging.; Cellular biology (cytology); Dermatology.; Ophthalmology.; Optical physics.; Fluorescence microscopy.; Lifesciences; Microscopes Collections: EBSCO Allowed Actions: –
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"This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology"--Provided by publisher.
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Table of Contents
- Preface
- Foreword
- Contents
- List of contributing authors
- Part I: Basics
- 1 Brief history of fluorescence lifetime imaging
- 2 The long journey to the laser and its use for nonlinear optics
- 3 Advanced TCSPC-FLIM techniques
- 4 Ultrafast lasers in biophotonics
- Part II: Modern nonlinear microscopy of live cells
- 5 STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities
- 6 Principles and applications of temporal-focusing wide-field two-photon microscopy
- 7 FLIM-FRET microscopy
- 8 TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing
- 9 Laser tweezers are sources of two-photon effects
- 10 Metabolic shifts in cell proliferation and differentiation
- 11 Femtosecond laser nanoprocessing
- 12 Cryomultiphoton imaging
- Part III: Nonlinear tissue imaging
- 13 Multiphoton Tomography (MPT)
- 14 Clinical multimodal CARS imaging
- 15 In vivo multiphoton microscopy of human skin
- 16 Two-photon microscopy and fluorescence lifetime imaging of the cornea
- 17 Multiscale correlative imaging of the brain
- 18 Revealing interaction of dyes and nanomaterials by multiphoton imaging
- 19 Multiphoton FLIM in cosmetic clinical research
- 20 Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery
- 21 Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models
- 22 Bedside assessment of multiphoton tomography
- Index
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